Canine_MUO_CSF_5 - SRA

SRX10000107: Canine_MUO_CSF_5
1 ILLUMINA (Illumina MiSeq) run: 1.1M spots, 454.6M bases, 259.2Mb downloads

Design: RNA was extracted with TRIzol (Thermo Fischer Scientific) and reverse transcribed to cDNA with non-ribosomal hexamers using Superscript IV (Thermo Fischer Scientific) using non-ribosomal hexamers. Second-strand cDNA synthesis and random amplification of samples was performed as previously described. A DNA library was constructed using the NexteraXT protocol (Illumina) with NGS performed on an Illumina MiSeq system using MiSeq Reagent kit V3 (300 x 2 cycles; Illumina).

Submitted by: University of Veterinary Medicine Hannover

Study: Canine Meningoencephalitis of unknown origin, the search for infectious agents via deep sequencing

PRJNA698629 • SRP304169 • All experiments • All runs

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Meningoencephalitis of unknown origin (MUO) describes a group of meningoencephalitides in dogs and cats with a hitherto unknown trigger. It is postulated that a known or unknown pathogen might be associated with the initiation and/or progression of this disease. In this study, we have performed next generation sequencing and analysis on fresh-frozen cerebrospinal fluid samples of six dogs with MUO in the acute phase of the disease.

Sample: Canine_MUO_CSF_5

SAMN17730368 • SRS8169883 • All experiments • All runs

Organism: canine metagenome

Library:

Name: NGS_16005

Instrument: Illumina MiSeq

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 1.1M spots, 454.6M bases, 259.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR13605964	1,122,333	454.6M	259.2Mb	2021-02-02

ID:: 13102589

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